Reeler Mouse
The Reeler mouse brain imaged with MRM at 21.5 mm
Three high resolution (21.5 mm) MRM brain scans for: a) a wild type control (WT), b) a heterozygous Reln mutant (Relnrl/+; c) a homozygous Reln mutant (Relnrl/rl).
Overview
The data sets consist of three high resolution (21.5 mm) MRM brain scans for: a) a wild type control (WT), b) a heterozygous Reln mutant (Relnrl/+; c) a homozygous Reln mutant (Relnrl/rl).
Figure 1. Magnetic resonance microscopy at 21.5 mm used to study the neuroanaomical phenotype for the reeler mouse (Reln). The ventricles (cyan) and hippocampus (yellow) were segmented in wild type (WT), heterozygous (Relnrl/+), and homozygous Reln mutants (Relnrl/rl). The ventricles and hippocampus are depicted in the first row for WT (Left), Relnrl/+ (Middle), and Relnrl/rl (Right) animals in the background of the brain. Enlarged ventricles can be seen for the Relnrl/+ and Relnrl/rl mice; while the hippocampus is smaller for the homozygous mutants. On the second row, horizontal sections through the brains highlight the layered structure of the hippocampus, and the ventricles. Gadolinium-based active-staining show that layers of the hippocampus appear disorganized in Relnrl/rl mice (Right). Also layers of the cerebral cortex can be visualized in the WT animal (Left) and the Relnrl/+ (Middle), but are not as easily seen in Relnrl/rl mice. The brain and the cerebellum size are noticeably smaller in the Relnrl/r l.
Description
Male and female adult WT, heterozygous, and homozygous mutant Reln mice (Jackson Laboratory, Bar Harbor, Maine, USA) were used for this study. The animals were approximately 5 months of age at the time of investigation. In preparation for morphology studies, mice were anesthetized with 100 mg/kg pentobarbital (i.p.) and transcardially perfused with a solution of 10% formalin and 10% ProHance (Bracco Diagnostics, Princeton, NJ) as described by Johnson and colleagues (Johnson et al., 2002). Heads were stored overnight in formalin and scanned the next day. The brains were placed in fomblin-filled tubes and were scanned in the skull to avoid distortions of brain or damage to tissue due to handlingImaging was performed on a 9.4 T Oxford vertical bore magnet with a GE EXCITE Console (EPIC 11.0) that has been specially adapted for MRM. Images were acquired with a radio frequency refocused 3D spin warp sequence with TE/TR of 5.1/50 ms, a field of view of 22x11x11 mm and zero filling was used, resulting in a 1024x512x512 matrix and an isotropic resolution of 21.5 µm. The sequence used non-uniform radial gain and asymmetric data collection resulting in a scan time of 2 h and 7 min per brain (Johnson, in preparation). One image set corresponding to one animal from each group (genotype) was included in the zipped archive available for download.
Usage
SHIVA or MBAT are recommended for visualizing the datasets.
Contributors
Alexandra Badea1, Peter J. Nicholls2, G. Allan Johnson1, and William C. Wetsel2,3
1Center for In Vivo Microscopy, 2Department of Psychiatry and Behavioral Sciences, 3Cell Biology, 3Mouse Behavioral and Neuroendocrine Analysis Core Facility, Duke University Medical Center, Durham, NC 27710, USA
References
A. Badea, P. J. Nicholls, G. A. Johnson, W. C . Wetsel. Neuroanatomical Phenotypes In The Reeler Mouse. Neuroimage. In Press.
Johnson G.A., Cofer G.P., Gewalt S.L., Hedlund L.W., 2002. Morphologic phenotyping with MR microscopy: the visible mouse. Radiology 222, 789-93.
Acknowledgement
This work was made possible through collaborations with the Mouse Bioinformatics Research Network (MBIRN), through NIH/NCRR grant U24 RR021760. All experiments were conducted at the Duke Center for In Vivo Microscopy, a NCRR/NCI National Biomedical Technology Resource Center, and were partially supported by unrestricted funds to W.C.W. and by grants P41-RR005959 and R24-CA092656 to G.A.J.